Dear all
I would like to measure transcript levels of S100A9
Can I do that using oligos that recognise UTRs (not for CDS)?
Thank you so much
Can I ship cells overseas on dry ice? Does everyone have experience on this? We are going to extract RNA and protein (not culturing) from the cells (Buffy coat). Thank you so much!
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Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...
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What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
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I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
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Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...
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I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
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What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
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I am struggling to attain multiple research articles, so far, I have looked at the Swedish longitudinal study, they have good depth and reliability. I was thinking of looking into twin studies to...
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I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
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I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
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