I am trying to investigate the effect of drug treatment on nuclear morphology by DAPI staining. However, I am yet to standardize the method and get good results. Can anyone share his/her protocol for the same? I will be thankful.
Hi Jay, with morphology you're talking about the shape of the nuclei itself or distribution within for example a tissue?
In the case of the nuclei shape I would suggest (if you don't have a special software for that) to use ImageJ and the analyse particles plugin. Besides the area you will also get shape-describing parameters like for example circularity.
Thanks a lot @Lukas Kristoffer Schwarz, I am interested in studying effect of some anticancer drugs on the nulclear morphology. As we know that DAPI binds to the A-T rich regions in DNA and shows fluorescence, it gives indication of damaged/intact nuclei. I wish if I could get a standard protocol regarding DAPI concentration, permeabilization buffer composition (Triton X concentration) and also incubation time for better results.
Thnaks for your interest!
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