I have followed the Tylosin extraction method from soil from paper for HPLC-UV analysis the extraction method includes adding 0.8 g Sodium chloride and 8 ml acetonitrile to 1 g of soil sample contaminated with Tylosin
My question is can I follow the same method to extract other antibiotics such as
Doxycycline Hyclats
Oxytetracycline Hcl
Sulfadimidine sodium
Can you use the same extraction technique for all of your compounds? Not enough information is known/provided. "HPLC" is the name of the just the technique. It must be defined. The same is true for the sample prep procedure. For any analysis to be valid, it must be combined with an HPLC "METHOD", fit for purpose, which is shown to be selective for the compound of interest and follows good chromatography fundamentals. *There is no "universal" antibiotic method. Review the different compounds of interest to find out which sample prep techniques are appropriate, then search for an appropriate method to use. The sample (*or sample mixture) should be analyzed using an appropriate method of analysis for all of the compounds present to insure good selectivity and accurate results.
- If you know the names of the various compounds you expect to find in the sample, then you can use a keyword search (using a search engine such as GOOGLE or Bing) combined with the keyword(s) "HPLC", method" and/or "Analysis" to find many example application notes, white papers, journal articles etc. This is often a good place to start and will provide you with some basic info to get started (saving time and money). If YOUR specific samples are in soil (the matrix), then look for articles which describe extraction methods and analysis techniques for soil samples.
Also, as you are new to liquid chromatography please request help from your teacher and/or a local chromatographer who is experienced using your HPLC system and software.
Yes you can by using the potent combination of 0.8g Sodium chloride and 8ml acetonitrile as your magical elixir. With a wave of your lab wand, you can unlock the secrets of Doxycycline Hyclate, Oxytetracycline HCl, and Sulfadimidine Sodium hidden within the soil samples.
After mixing. The HPLC-UV analysis will act as your enchanted mirror, revealing the chemical fingerprints of each antibiotic, like a treasure map guiding you to their distinct peaks.
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