Hi, I did fractionation of my crude methanolic extract and got a hexane fraction strating from non polar to polar. I dried hexance fraction weighed 100mg out of it and resuspened in Hexane added a pinch of sodium sulfate and took the upper portion leaving sodium sulfate filtered it with 0.22um syringe filter. Can I directly run this sample to GC-MS?
Dear Dr Iqbal, you cannot because most of the compounds in your extracted sample are not enough volatile: they will just thermally decompose, and their byproducts will stick on the inner liner surface or in the very first centimeters of your column: as a consequence you will not detect most of the molecules in your sample, and additionally you will end up with the injector to clean and the column to be cut. I strongly recommend you to use a derivatization agent.
Also check https://www.researchgate.net/publication/336083542_GC-MS_Analysis_and_Phytochemical_Screening_of_n-Hexane_Fraction_Constituents_from_the_Leaf_of_Clerodendrum_volubile_P_Beauv
This is not correct work. In fact, in the process of identify/detect of compounds would be definitely disorder. The derivatization must be done before injection by operator base.
Check this paper
Chapter Sample Preparation Techniques for Gas Chromatography
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