Yes
When preparing samples for transmission electron microscopy (TEM), it is crucial to use proper techniques to ensure accurate results. Diluting a bacterial sample with distilled water can be done, but it needs to be done carefully and with consideration of the specific requirements of your experiment.
you will dilute a bacterial sample with distilled water to prepare TEM sample. you must follow the steps:
a. Centrifuge the bacterial suspension to yield a solid mash.
b. the solid mash dispersed in distilled water as per requirement and again need to dispersed two drop suspension again add distilled water, follow the steps as per requirement of dilution.
c. then the sample transfer onto the grid and wait 1 day in a desiccator. after that insert the sample into TEM.
It’s significance carry out each step, every single steps can impact the quality of your sample.
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