I plan to isolate breast cancer cells from patient blood samples and would like to fix and stain them in-situ, within the Eppendorf, and then spin them onto a (poly-l-lysine-coated) glass slide for imaging. Has anyone done this? I cant see any protocols where staining and fixation has been carried out prior to cytospinning.
Following cytospinning, should i simply mount a coverslip onto the sample with e.g. DPX? Or should i clean with alcohols first?
Thank you for any advice you can offer!
Gail Sudlow
Why not just split your sample and use half for each or whatever proportion you need?
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Gamal Abdul Hamid
I think the idea by Gail Sudlow is the easiest and best method to avoid contamination.
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