Can I correct my biofilm absorbance reading results after using wrong wavelength?

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Is there anyone can help me with a software that can analyse seismic data acquired using Teraloc MK8?

I have already collected seismic data and need to analyse them. Please assist with the software that can also analyse the Peak Ground ACCERATION (PGA).

10 August 2024 4,839 0 View

What is the potential role of citizens in making public policy?

I am a student of public policy studying the policy cycle. I am curious about the potential role of citizens within this cycle as well as critique about the current situation I am writing from New...

03 March 2024 8,255 9 View

Does anyone have experience with RNA isolation from dead plant tissues like roots?

I have tried isolating RNA from dead roots with no success. We call them dead just because they are discolored and black/dark brown but not sure if that means they are completely dead. I have...

31 January 2024 5,300 4 View

Are there any volunteers age group (22-35yrs) and age group (36-60) to participate in a focus group discussion on smart services research?

Kindly contact me if you are willing to participate ([email protected]). Your participation will be greatly appreciated.

28 September 2023 7,054 0 View

Ex vivo PBMC media changes?

I am planning an experiment where I stimulate ex vivo PBMCs in plates for seven days before analysis of the T cell proliferation. This appears to be a pretty standard experiment, however, I can't...

27 September 2023 8,721 2 View

BOD titration. We add the manganese sulfate, the sodium iodide azide and concentrated sulfuric acid, but the solution goes colorless. Why?

We doing this as a high school project of BOD on pond water and are following a video method (https://www.youtube.com/watch?v=QZXlIbeGWA0) we found on the internet and we get a pale brown ppt...

27 September 2023 8,477 1 View

What is the impact of local labour market conditions on social assistance caseload?

We want to understand how local labour market conditions affect social assistance caseload, earnings and exits to employment in Ontario.

26 September 2023 3,570 1 View

CD5bright CD8+ T cells?

Does anyone have any papers characterizing CD5bright CD8+ CD4- T cells? I'm working with canine peripheral blood samples and noticed a distinct population but I can't find any discussion in the...

06 September 2023 8,629 0 View

Increased Metabolism in Cell Cultures Exposed to Stressor?

Is it plausible that cells exposed to an oxidative stressor would display increased metabolism (as measured through MTT assays)?

25 June 2023 7,034 2 View

Any appraisal instruments for non-clinical practice guidelines recommended?

Most studies discuss clinical practice guideline development process, of which, the tool can be used to appraise clinical practice guidelines. However, I seem to not get quality assurance...

18 June 2023 8,594 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View