How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Organofluorine compounds in Kombucha?

Kombucha, a fermented Tea with low pH, contains substantial Fluoride in the form of Hydrogen Fluoride as well as the ion. Kombucha has been associated with many adverse effects, including acute...

12 February 2020 408 1 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

What are the neurotoxicity biomarkers and parameters for study chronically and low dose of xenobiotics ?

i'm working on amelioration of neurotoxicity produced by long term and low dose of exposure of xenobiotics , please help and provide any links and papers.

06 July 2019 6,223 4 View

Which cell line from this list is the best to use in a neurotoxicity model?

The available cell lines are: (LAN1, LA-1-55n, LA-1-5s, SK-N-LP, SK-N-JD, BE-2C) which one of them can I use in Neurotoxicity model?

29 March 2019 4,670 3 View

Considerations in using SH-SY5Y cells for toxicology studies; which population of cells do we consider for assays- floating or attached or both?

In our lab, we are trying to understand the mechanisms by which certain xenobiotic compounds induce neurotoxicity using SH-SY5Y cell line as a in vitro model. As of now, we are encountering a...

11 December 2018 5,854 1 View

Considerations in using SH-SY5Y cells for toxicology studies; which population of cells do we consider for assays- floating or attached or both?

In our lab, we are trying to understand the mechanisms by which certain xenobiotic compounds induce neurotoxicity using SH-SY5Y cell line as a in vitro model. As of now, we are encountering a...

03 December 2018 6,055 1 View

Is Neuro-2a a cancerous cell line?

Hi All, According to ATCC, Neuro-2a is a Mus musculus brain neuroblastoma cell line. Further search totally confused me, because this cell line previously used to study neurite outgrowth,...

08 September 2018 7,941 4 View

What is the most efficient, least toxic vector for shRNA delivery to the brain?

I would like to knock down some proteins in the brain. I have used before siRNAs + JetSI transfection reagents for transient knock downs, but would like to do something longer lasting. I've seen...

06 July 2018 9,177 1 View

Non-neurotoxic brain dye for infusion?

Dear all, I'm looking for a non neurotoxic brain dye that I can infuse into the ventricles through osmotic pumps, to be sure that I'm doing the infusion in right way. The mice must survive for at...

29 August 2017 5,509 3 View

Non-neurotoxic brain dye for infusion?

Dear all, I'm looking for a non neurotoxic brain dye that I can infuse into the ventricles through osmotic pumps, to be sure that I'm doing the infusion in right way. The mice must survive for at...

07 August 2017 1,416 3 View