I'm planning to use MPTP and SHSY5Y cell line to model the PD in-vitro. but MPTP will not be metabolised to its neurotoxic form MPP+. Is there any way to do this step to prepare MPP+ then add it to the cells?
Personally, I wouldn't work with MPP+ for safety reasons.
In any case, MPTP can be converted to MPP+ by treatment with monoamine oxidase B. One commercial source of MAO-B is here, but it is very expensive.
https://www.sigmaaldrich.com/US/en/product/sigma/m7441?gclid=CjwKCAjwqvyFBhB7EiwAER786dt1Nw791DOEIbWEodMT9vXRR5BLSFxNN5PIZ3cZvHqe5RtcUnZCMRoCHzEQAvD_BwE
It would be much cheaper to buy MPP+ (but please be careful):
https://www.sigmaaldrich.com/US/en/product/sigma/d048
Hi, you can directly purchase commercial MPP+ and add it to your neuronal culture at appropriate concentration.
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