I get that the point of separating sterilization of YNB (or any amino-based compound) with sugar since it may result in Maillard reaction thus producing side product that may affect microbe growth.
But is it okay to autoclave YNB? Some source said that I may but some other prefer to use filter.
J. C. Tarafdar
@ Royyan, my suggestion is do not autoclave. Sterilize the solution by filtration and store at 2ºC to 8ºC.
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Harshit Gupta
@royyan Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.
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Chuck A Arize
https://www.thermofisher.com/order/catalog/product/Q30007#:~:text=Yes%2C%20YNB%20medium%20with%20ammonium,can%20be%20autoclaved%20for%20sterilization.
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