Yes, urine samples can be analyzed for polycyclic aromatic hydrocarbons (PAHs) using GC-MS without derivatization by employing methods like automated microextraction or enzymatic hydrolysis. These approaches simplify sample preparation while maintaining accuracy and efficiency.

Please remember that you should look more for mono-hydroxylated metabolites of PAHs than for the parent compounds (which can be, of course, be identified without derivatization or enzymatic treatment). Methods are already available for both GC/MS and HPLC/MS, see:

DOI: 10.3390/ijerph192013089

DOI: 10.1007/s00216-021-03638-4

DOI: 10.1016/j.envint.2021.106720

Yo do not need to derivatize... you may need to clean up your sample.