Can I add specific regulatory sequence in 35S promoter to make it inducible?

12 December 2016 1 2K Report

I need to make a promoter in a way that it contains specific regulatory motifs. Is it possible to add such sequences in 35S promoter and it will become the inducible promoter?

Rakesh Kumar Sinha

What I understand according that, you can try to add inducer sequence like VP64 /VP16. But the same time you need to insure where to add. Below publication might be helpfull.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/

http://www.jbc.org/content/280/5/3707/F1.expansion.html

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