Kindly explain the method to identify the species (Hook worm) on stool examination
Dear Vinoth Boopathy
The specific identification of hookworm eggs is not possible, since the eggs of species of Ancylostoma and Necator are indistinguishable. One possible form is to perform coproculture to obtain filarioid larvae (L3). The larvae of species of hookworms have morphological differences (e.g. shape of tail) that make possible the specific identification.
Best regards
Hudson
Yes it is difficult to differentiate hookworm eggs via stool examination
We once did this a complicated way, using eggs from stool specimens. The title of the following paper is self-explanatory: Evans AC, Daly TJM & Markus MB. 1991. Identification of human hookworm in failed-treatment cases using Chinese hamsters (Cricetulus griseus) and scanning electron microscopy. Journal of Helminthology 65: 67-72.
With usually coprological technique its no possible to identified Ancylotomum from Uncinaria or Necator. Markus describe really complicated way and I don't do it without good reason. Coproculture was easier way. Eventually we may made difference of eggs at fresh stoll sample which we frozen at -20oC and after few day we put stoll at water and after they defrost in the water let it sediment and examined.
With reference to Ivan's comment, it was of course not a technique that we used for routine diagnosis.
I agree with Hudson Alves Pinto. The best way to identify hookworm species is by means of coproculture. The method (Harada Mori technique) allows the identification of filariform larvae by examining their morphological features.
Yes, no way to differentiate them just by egg observations. But, depending what is your aim, the number of samples and how your lab is equipped and you are familiar with molecular technics, it could be more easy and quickest to use some published PCR than coproculture.
My dear Vinoth.
For laboratory diagnosis of worms it is necessary to make a worm eggs in stool, through spontaneous sedimentation (HPJ) the MIFC, Willis method, among others. To do research larvae, they are used methods of Baerman - Moraes or Rugal method.
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