maybe you can incorporate skim milk or prebiotic substances it might improve its viability during expose to heat

If they are truly killed with 100% efficiency, then of course they cannot grow. However, if some cells have survived, then they can potentially grow. In particular, with radiation, if a small percentage have survived, then they may grow, but the ones that do grow may be significantly mutated from the original strain. So the short answer to your question is NO, by definition of "killed".

Please mention why you want to colonize Lactobacillus sp. in the human intestine through oral administration, given the fact the killed bacteria won't be able to secrete any antimicrobials unless it is meant to occupy the available spaces in the lining.  

Heat 121 Celsius temperature and radiation both kill the Lactobacillus sp. but human body can not  tolerate this much heat and radiation also harmful in high amount.  

Well thanks to everybody for your responses. I want some lactobacilli which will colonize in human intestine. But definitely will not grow there. It may stay there for some time like 1 week or so.

Prof. Cote I totally understand that when something is dead is dead. I am wondering if we have any known instances where after killing those bacteria they were able to colonize in human intestine due to intact cell adhesion molecules like cell surface proteins etc

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Dr. Karim, yes I want the killed cells (attenuated)  to occupy the lining and stay there for some time period like for a week. Do you have any idea. Please share

To attenuate lactobacillus is better to apply heat. Radiation change the origin strain. To colonise  the protein should have some biological activity. If killed, dont think so can do this. Also the the gut there are so many changing, so bacteria to survive should be replicate. If want to check adherence properties is better to use live lactobacilus and after take a SEM photo or just simple Gram stain. 

I think the killed cells start lysing(depend on the method of killing) and I don't think their adherence property remain the same. On the other hand, cells need to contact with epithelial cells or mucus layer depend on the host for Lactobacillus.  Lactobacilli will  colonize of its own host usually.They need to devid, make layers and establish a biofilm there.Oral administration of lactobacilli with human origin  in rodent can stay in gut for at least less than a week(depend on the stain), but at the end all the bacteria will shed out eventually.

Thank yo everyone for the inputs, I welcome the suggestions. sterilization defines complete death of cells, are there any chances that the DNA of dead cells contribute for recombination with the intestine colonized lactobacilli via transformation, such instances are reported in the past? Thank you.

could you please give a reference Shankarappa?

Thanks everyone for your valuable comments. 

Dr. Karimi, to achieve my goal I need the bacteria to adhere on the intestinal lining but at the same time I can't allow them to grow there. Otherwise my whole purpose will defeat.  

HI Diptiman,

In the GI tract, there is competition for niches and nutrients  for commensal bacteria.( It is not friendly environment  for all).The transient bacteria (experimental bacteria in your case) should be able to compete with those that  already  well adapted to their host and posses their niches in the lumen, therefore most of transient  will be out compete and can not  divide (in their full capacity) due to unfriendly environment ,thus will stay short period of time and then pass through. 

when killed they or cell wall matrial may adhere to intestinal cell walls or mucose for a time but in general as not alive the cells will be washed through, still they may though have immunological effect as several studies have shown!

 Hi Per,

Thanks for your comments. Do you have any reference for that. Please send me one if you find.

Hi Dr. Karimi,

Thanks for comments. Let's see how does it work for me.

There are several Japanese companies, most known is Calpis, that sell like Lb.acidophilus heat treated dead cells as probiotics that have proven immonological effects from clinical trials, though all cells dead and they therefore naturally can not colonize! I have seen some of their data, but if published anywhere??