Hello everyone, I need to tag late endosomes, and I have FITC-Dextran of molecular weight 70KDa, can it be used to pulse chase phagolysosomes? Also how long should I treat THP1 cells with FITC-Dextran to tag the phagolysosomes?
01 February 2017 2,080 7 View
I have to infect them in 24 well plate for an experiment but I see they are already overcrowded (almost confluent), have to do LDH assay after 24 hrs., would it affect my results? Should I go on...
06 July 2016 3,983 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View