Mouse brain extraction without fixation perfusion?

I am currently working on a mouse model that I will be collecting long bones and muscles from. We have decided to extract the brain for IHC and we do not want to perform transcardial perfusion as...

12 November 2020 5,821 4 View

How do we measure collaborative writing motivation?

I am conducting a study on collaborative writing using an experiment (pre- and post tests). How can I measure students' writing motivation? i mean if they are more motivated or not.

30 March 2019 6,739 3 View

Which type of solar system is better to integrated with solar water heater?

Which type of solar system is better to integrated with solar water heater?

23 December 2018 6,969 8 View

When is the best timing to include treatment using soft agar colony formation assay?

We often use soft agar colony formation assay to test cancer cells' Anchorage-independent growth as a hallmark of carcinogenesis, the assay described by "Stanley Borowicz, et. al.". Yet, I can...

13 October 2018 5,241 3 View

Is it possible that bacteria or virus might be one of diabetes causative agent and can transmit from one to another?

27 September 2017 4,149 4 View

What are the compatible solutes for HPLC c18 reversed phase columns other than toluene?

What are the most common among them? Also what are their operation conditions?

21 April 2015 7,603 10 View

How do I design an optical gate for demultiplexing OTDMA signals?

Hello everyone, I am preparing to simulate a Fiber To The Home FTTH system. My problem is related to the implementation of multiplexing the upstream from the users (ONUs) using OTDMA. At the OLT,...

06 September 2014 5,943 7 View

Is there any way to implement the MATLAB code in the Raspberry Pi device to make it work as stand-alone?

Hello... By using MATLAB R2016a and Raspberry Pi 3 Model B, I have designed a device that can be used to detect drowsiness by analyzing the facial expressions (High-Level Design Attached). The...

01 January 1970 315 5 View

Is aligning mitochondrial and nuclear sequences inconsequential?

Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...

19 January 2021 5,895 2 View

How to calculate sample size for the study of disease prevalence ?

Currently planning to work to find the prevalence of hydatidosis among domestic cattle in remote areas. The previous prevalence rate was unknown as no research was conducted yet in that area.

24 November 2020 9,598 4 View

Matrigel dissolves during paraformaldehyde fixation?

Hello, I am working on 3D culture of MDCK cysts. Whenever i try to fix the cells with PFA, the matrix depolymerizes. We thought it was something to do with the matrigel, so we tested different...

01 February 2020 5,875 2 View

How to induced toxoplasma cyst in vitro?

I am trying to induce toxoplasma cyst formation (both RH and ME49) strains by altering the pH to 8.1 (DMEM, 1%FCS, 1% pen-strep), at 37oC, without CO2. However, the host cells weren't healthy and...

10 November 2019 9,882 1 View

35 year old women presented with central periumblical pain specially with each menstrual cycle.What is the provisional diagnosis and definitive R ?

She had 3 children and previous history of ceasearian section. on abdominal US there is left ovarian cyst what is the provisionsl diagnosis and definitive treatment?

11 December 2018 2,379 0 View

How to lyse the wall of Gardia cyst?

I am trying to extract Gardia DNA from stool. What I succeeded to get so far is in range of 1000 bp which is far to short than published. Any suggestions?

09 October 2018 5,636 1 View

Can ELISA be used to measure cyst fluid tumor markers?

In reviewing methodology of a manuscript, I found out that CA125 levels in ovarian cyst fluids were measured using an ELISA kit. In the kit manual, it says that this kit is used for measuring...

08 August 2018 1,547 3 View

Can ELISA be used to measure cyst fluid tumor markers?

In reviewing methodology of a manuscript, I found out that CA125 levels in ovarian cyst fluids were measured using an ELISA kit. In the kit manual, it says that this kit is used for measuring...

07 August 2018 7,010 4 View

I am interested in learning about effective interventions for severe buttock pain due to Tarlov cysts?

Any information is helpful!

05 June 2018 7,298 0 View

What are the ways for controlling brain bulge during intra-operative period during the intracranial surgery?

Intra-operative bulge of brain during intracranial surgery is a devastating situation, many measures are employed in the graded ways to immediately control , other wise, it can lead to rise in...

04 May 2018 9,980 0 View