Yes, it is possible to extract DNA directly from kombucha using the Wizard kit. The key steps involve pelleting the microbial cells from the liquid using centrifugation, performing thorough lysis, and then following the standard protocol for DNA purification. If needed, consider additional pre-treatments like filtration or mechanical disruption to optimize the extraction process. Ensure that you evaluate the extracted DNA for quality before moving forward with sequencing or downstream applications.

Thank you so much for your answer Nicolas Poirier I'll try it!

Yes, DNA can be extracted directly from kombucha using the Wizard® DNA purification kit (e.g., Wizard® Genomic DNA Purification Kit by Promega). Kombucha contains a complex microbial community, including bacteria and yeast, embedded in a cellulose matrix (SCOBY). Extracting DNA from this mixture can be challenging, but it is possible with proper preparation.