I have three kelp, two green, and one red seaweed species that I would like to analyse and quantify for their fatty acid content. I do not intend to differentiate between lipid classes. Would a direct methylation approach be suitable for this purpose? I plan to use freeze-dried, powdered seaweed and extract the lipids using 0.5 M NaOH in methanol, with C21:0 in chloroform as an internal standard. For methylation, I will use 5% sulfuric acid in methanol and heat the mixture at 70 °C for two hours. The resulting FAMEs will then be extracted with hexane and washed with saturated sodium chloride solution to remove residual aqueous components and impurities. Your feedback on this approach would be greatly appreciated.