I am working on highly basic and hydrophobic protein that don't have any cysteine residue but highly express in dimer form.I want to convert that dimer protein into monomer form.
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07 August 2024 7,013 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
For Example, Company A & B merged with Company C , so during the pre merger period there are 3 company so how to do its pre merger analysis with post merger company? (either its 1 to 1 or 3 to 1)
31 July 2024 2,503 3 View
I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me
30 July 2024 3,743 0 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View
I forgot to autozero during the run (Size exclusion chromatography.) and later i realised i forgot to do that and the baseline was not zero but below zero (and in some cases it above zero). I...
15 July 2024 5,551 6 View
I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...
15 July 2024 5,157 2 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
PhD in Social Entrepreneurship and Digital Technologies
04 July 2024 1,675 2 View
I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...
16 July 2024 2,757 2 View
As, my studies are related to water adsorption, the substrates should be hydrophilic in nature. Ni-foam being hydrophobic in nature, the results are not as expected as in literature. Hence i...
14 July 2024 8,847 4 View
Suggest some procedure where i can synthesize [Ru(p-cymene)Cl2]2 in a good yield.
23 June 2024 9,244 2 View
Why DMSO is mostly used as a solvent for hydrophobic substances for cell study or any other biological study rather than ethanol or any other organic solvent
06 June 2024 7,020 2 View
Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...
21 May 2024 9,285 3 View
Hi, I ahve tried dna extraction and when I load DNA in agarose, I have seen dimer like dimerprimer in PCR, can anyone tell me why I saw dimer in dna extraced?
07 May 2024 9,269 4 View
Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...
29 April 2024 6,779 1 View
Hallo everyone! Need an advice, in an experimental setup. In brief, I study a protein which undergoes a proteolytic processing. It is known that the protein is present upon physiological...
13 April 2024 1,703 1 View
I have deposited chrome on the glass wafer. Then I applied a dielectric layer of SU-8 over it. After that to make the surface hydrophobic, I applied Teflon by using spin coating. However, I found...
12 April 2024 7,009 3 View
Hi all, I'm Dabin Xie from Zhincai Tech, a small research-driving company in China. I'm currently trying to modify glass with a single layer of silane whether hydrophobic or hydrophilic, like...
08 April 2024 2,628 4 View