I feel a little confused because calcofluor white can bind to cellulose and chitin, however, biofilm matrix of P.aeruginosa contain mostly alignate, PsI and PeI polysaccharides.
Dear Xinyi Pang, I wouldn't have thought that using Calcofluor would be very helpful in the visualisation of P. aeruginosa biofilms. Calcofluor has proven to be very useful in our surveys of fluorescent pseudomonads and even low levels of cellulose-expression are detectable with a fluorescent microscope, whilst higher-levels of expression can be detected using Calcofluor plates and a UV light source. In these surveys, we have yet to find a P. aeruginosa strain that expresses cellulose. You might find it better to visualise P. aeruginosa biofilms using a DNA stain by microscopy, and a general vital stain like Crystal violet if you are using microtitreplates or microcosms. Regards, Andrew.
Thank you Andrew. If I just want to observe the EPS of P. aeruginosa, what dye should I use? I notice some papers use it to stain the biofilm matrix of P. putida.
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