Hi,
for my research I need to use laminin for some patterning and am wondering whether the C17.2 cells (neural progenitor cell line) be cultured on it (instead of poly-l-lysine as suggested by the distributor and papers).
The first test schows that they do attach without problems, but is there a risk that they will they de-differentiate?
Would be grateful for any experience with these cells or other neural progenitor cells regarding the coating!
Terrance Grant Johns
The cells will certainly grow on laminin but it will change their biology. The answer depends on what experiments you are planning. I would stay with the suggested approach.
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