I am having problem with quality of amplification of my Mangrove DNA samples. Can I use these two adjuvants for improving the quality of PCR amplification!!
yes, you can use both in reaction, but problem may be related with DNA quality or primers or PCR conditions.
Use DMSO first, along.
Check the DNA quality and quantity. And also try to optimize the PCR conditions.
Most likely your problem is in the primers and polymerase. You should choose again primers and try Taq-poly another supplier (e.g. Qiagen, Invitrogen etc.) or try another DNA-poly (e.g. PFU). If you will use Taq then you should optimise magnesium concentration in Mg-gradient and optimise Tm of your primers.
There are several well-known additives for PCR-improving, but this improvement is not so critical in comparison with primers changing.
Some good articles about the additives:
Trehalose: http://www.sciencedirect.com/science/article/pii/S1525157810600438;
http://clinchem.aaccjnls.org/content/50/7/1256.long
TMA chloride: http://nar.oxfordjournals.org/content/18/16/4953.full.pdf+html;
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC307197/pdf/nar00016-0271.pdf;
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528177/
DMSO and Betaine: http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0011024
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