Does anyone simulate the supercooling phenomenon of the PCM (COMSOL)?

I have a heat transfer model in the thermal bath (temperature changes in the range 23-33degC), in which a vial with PCM material is immersed (melting temperature is 28degC). During the heating...

03 January 2021 3,548 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

16 November 2020 2,373 3 View

How to obtain promoter sequences?

Hi dear fellows, I would like to perform ChIP-PCR on the promoter sequences of genes, but I am not quite sure how to design primers for the promoter sequences. Can someone please help me? My...

20 September 2020 7,371 6 View

Recommended packages or references to combine regional climate models and Holdrige life zones with R?

I want to use R to make regional climate models to analyze changes in Holdridge's life zones. I haven't used R in a long time so I'm kind of outdated and would like to read some references that...

01 July 2020 1,728 4 View

What strategies to use to make distance classes more attractive to students?

In times of pandemic, distance learning has gained strength. What can teachers do to make distance classes more attractive to students?

23 May 2020 8,148 22 View

In your opinion, are bibliographic review articles important and should they be published by scientific journals?

Some journals do not accept review papers because they consider that the theoretical framework is the basis of a research, and not the research itself. Other magazines already accept and encourage...

20 May 2020 7,841 50 View

Metabarcoding fungi in lake sediment cores - advantages/disadvantages of ITS and 18S?

In my PhD, I want to analyse fungal communities in lake sediment cores from Siberia. One of the performed experiments will be a metabarcoding of the fungi. Therefore I am evaluating primers at the...

05 May 2020 3,920 1 View

Which species do you recommend for ecotoxicological tests of industrial effluents containing hydrocarbons?

The question aims to know what types of species are most used in ecotoxicological tests of effluents from industries in the oil sector.

26 April 2020 5,382 21 View

How can I include a second order term in the model?

My hypothesis is that the functional diversity of fish (responsible variable) increases until an optimum level of a gradient of habitat structural complexity (predictor variable), but decreases...

01 April 2020 9,569 4 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View