I want to nucleofect 293T cells using AMAXA Nucleofection kit, but the website shows the optimized program only for 293 cells. Does anyone here use AMAXA kit?
I am trying to optimize Nucleofection to MEFs using home-made buffers, since the buffers from the company are expensive. Could anyone please help me with this? I would be very grateful if you did.
03 April 2014 1,464 2 View
I made SDS Gels according to a recipe I found online. Most of the time the gels are fine, but in some cases the wells leak. I always remove the combs upright, but could there be any other...
02 March 2014 3,465 8 View
I have noticed that the aliquoted diluted dNTPs as they age stop giving results for PCR. Do anyone know the reason for this or have experienced the same? For some reason I think that they retain...
10 November 2013 6,844 7 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
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In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View