Yogesh HN

4 Questions 1 Answers 0 Followers

Questions related from Yogesh HN

Does anyone have a home-made recipe for Amaxa Nucleofector Kit 2?

I am trying to optimize Nucleofection to MEFs using home-made buffers, since the buffers from the company are expensive. Could anyone please help me with this? I would be very grateful if you did.

04 April 2014 1,430 2 View

Can anyone tell me which program to use for AMAXA Nucleofection kit for 293T cells?

I want to nucleofect 293T cells using AMAXA Nucleofection kit, but the website shows the optimized program only for 293 cells. Does anyone here use AMAXA kit?

04 April 2014 6,979 0 View

Why do my samples leak after being loaded SDS-PAGE wells?

I made SDS Gels according to a recipe I found online. Most of the time the gels are fine, but in some cases the wells leak. I always remove the combs upright, but could there be any other...

03 March 2014 3,430 8 View

Why does PCR fail with dNTPs as they get old?

I have noticed that the aliquoted diluted dNTPs as they age stop giving results for PCR. Do anyone know the reason for this or have experienced the same? For some reason I think that they retain...

11 November 2013 6,805 7 View