By removing the seed hull gives effective breaking seed dormancy.

one method - Heat treatment (50 C) for 7 or 14 days.

another method by chemical treatment by using

a. 0.001 M potassium nitrate (KNO3),

b. 0.1 M and 0.2 M nitric acid (HNO3),

c. I M hydrogen peroxide (H2O2),

d.1000 ppm gibberellic acid (GA3).

treat seeds for 24 h - 48 h.

I worked with O. sativa and de-hulling is effective in breaking seed dormancy. I have experience in chemical scarification with pure sulphuric acid to break dormancy in Echinochloa spp. Some biotypes need to be soaked in H2SO4 even for 30' minutes. It could work also with the two species you are testing.

thanks adams and silvia. i tried the method of GA and HNO3 but with no result. i will try the rest of the methods and let you all know the results.

any more suggestions please..

in Indonesia we traditionally using floor heat direct the sunlight, and cover it over the night. maybe the treatment are: HEAT and MOIST. after 2-3 day then put into plastic bag and submerged it into the water for 1 night (observe the last step, and repeat)

Heat treatment in 40 degree centigrade, in 100 RH might, 8 hours, is enough to generate seed embryo to grow

Seeds may be soaked in ABA solution for overnight to break dormancy, the concentration of 0.5, 1.0 and 2.0 ppm can be tested and the one with best results may be selected.

dear singh

as far as i know, ABA is a hormone that induce dormancy. can you please let me know of any literature that studied the effect of ABA on dormancy breaking?

Dear Prasad,

yes, u r right, it was by mistake, it is GA not ABA.

Dormancy in most rice varieties can be broken through, heating, treatment with nitric acid and dehulling. Try priming the seeds too

Dormancy in most rice varieties can be broken through, heating, treatment with nitric acid and dehulling. Try priming the seeds

Remove the seed hull, scratch the seed coat near by embryo, and culture in MS medium

try conc. H2SO4 for about 10min scarification.

Dear Mr. Prasad,

First, incubate the seeds (Hulled and de-hulled) at 50 degree C for 48 hours. Then put in pettridishes with cotton for  more than 96 hours. I have found 98% germination. Please proceed.

You can also incubate seeds with 1mg/ml GA3 for 48 hours followed by heat with waterbath for 15 mins. Then soaked in water with cotton in pettridishes for 4 to 5 days. After every 12 hours 0.5ml of 0.5mg/ml GA3 can be applied. Currently i am experimenting on this method. You can also do the same.