Can anyone tell about the separation methods for 3 to 10 kDa peptides from crude protein?

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Kamran Pooshang Bagheri

You can use ultrafiltration methods.

by using a 10kda filter, the peptides pass through and the proteins remain backward.

Good luck

Carlos Enrique Perez Heredia

In dependency of total volume to work. For pilot scale or same, I propose use UFT with membranes (8 – 10 kDa), in Vivaflow or Sartocon Slice (200) system. For lab scale, I propose HPLC separation (SEC or RP). Regards for all.