Separation method for single peptide (below 10kda) rather than SDS and reverse phase HPLC.
You can use ultrafiltration methods.
by using a 10kda filter, the peptides pass through and the proteins remain backward.
Good luck
In dependency of total volume to work. For pilot scale or same, I propose use UFT with membranes (8 – 10 kDa), in Vivaflow or Sartocon Slice (200) system. For lab scale, I propose HPLC separation (SEC or RP). Regards for all.
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