Labeling of Protein with Dye?

Hello Dear All, I have labelled a protein with dye. Dye has excitation/emission spectra around 800 nm wavelength, i.e. NIR region. I want to check whether there is some free dye present or Not?...

02 March 2020 8,894 3 View

Crosslinking molar calculation???

Dear All, I want to ask about some molar calculation of networks formed through esterification. First, I have a linear polymer chain with single pendant hydroxyl grp. Second, i have a diacid...

11 December 2018 4,804 3 View

Catalysts for Steglich Esterification?

Hi, Can somebody suggest, How much equivalent of DMAP should be used in addition to EDC for esterification?

09 October 2018 2,583 8 View

Polymer Solvent interaction parameter for a cross-linked network?

Dear All, I want to measure polymer solvent interaction parameter for a cross-linked network. Could someone please explain, How can I do it? Kind Regards

06 July 2018 2,088 9 View

Purification of Hydrogels?

Hi Guys, I am working with purification of gels, by washing with some solvents, so that, impurities get removed. But while doing that, I am getting some cracks after washing. I have fabricated...

03 April 2018 9,427 8 View

Protection of Phenyl boronic acid?

Hi All, Could some one suggest an easy and efficient way to protect phenyl boronic acid with ethylene glycol? Kind Regards

01 February 2018 4,824 12 View

Alternative for EDC, DMAP as catalyst for carboxylates?

What can be the best alternative for EDC, DMAP as catalyst for carboxylates? I want to skip DMAP actually.

01 February 2018 8,057 12 View

Grafting of Phenyl Boronic Acid with Polymer having pendant hydroxyl group?

Dear All, I want to graft PBA (Phenyl boronic acid) with some polymer having pendant hydroxyl groups. From Previous literature and my own observation, boronic acid forms a complex with these...

31 December 2017 6,093 2 View

Double Dynamic crosslinking?

Could some one please explain double dynamic chemical crosslinking? Thank you.

31 December 2017 7,514 0 View

Boronic acid and Polyesters?

Hi Dear All, Boronic acids in general form complexes with cis 1,2 or cis 1,3 diols etc. If we want to protect the boronic while attaching it to any polyesters with multi hydroxyl groups. Could...

31 December 2017 8,859 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View