Can anyone suggest stereotaxic coordinates to target the primary auditory area in postnatal day 21 mice?

29 June 2021 1 6K Report

I am aiming for L5 neurons. I am not sure whether to scale from adult coordinates or if there is a good p21 mouse atlas online.

Hadi Jabbar Alagealy

you can Find coordinates from Franklin and Paxinos atlas.

The Correct coordinates for fixation (the slides in the Paxinos atlas have been fixed and so shrunken by around 2-4%.however, Identify Bregma and Lamba in mice and calculate distance. Normalise coordinates according to B-L ratio with the idealised version in the Franklin & Paxinos atlas. Note also, that different mouse strains tend to have slightly different brain coordinates, so there will be an element of trial and error involved.

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