Regarding metagenomic contamination?

We have lab established culture setup of marine synechococcus PCC 7002 sp. and heterotrophic bacteria. Recently we did metagenomic analysis of it (sequencing was done by an outside reputed...

12 August 2020 187 11 View

How to find complete protein sequences (not partial) from NCBI database?

I want to generate a custom database of translated proteins involved in a particular process and then blast them against my predicted protein sequences from the metagenome . I have downloaded all...

12 July 2020 2,651 1 View

Can we collaborate on ongoign COVID-19 projects?

Hello all, I am a clinical epidemiologist with training in data analysis, manuscript writing and systematic review. Is there any group around where we can collaborate on your ongoing...

08 June 2020 3,831 48 View

How to identify a particular gene/gene cluster of interest from a sequenced genome?

We have sequenced Synechococcus sp. PCC 7002 genome. Already the same strain has been sequenced and annotated by many before. Whole-genome map is also available online. The gene of interest is...

17 March 2020 898 12 View

Any online tool or software to compare a FTIR spectrum provided in PDF format?

I have an FTIR spectrum of an exopolysaccharide. It is in a PDF file and not done at my lab. Is there any software or tool which I can use to compare with an existing spectrum of previously...

31 July 2019 6,257 2 View

Which is the best/optimum preservative techniques for RNA preservation?

we want to carry out transcriptomics study of a mixed marine bacterial community. The experiment is designed as such that we have to preserve the samples for later transcriptomic studies...

26 February 2019 7,220 16 View

RNA extraction and gel electrophoresis ?

Dear all, I am trying to isolate RNA from bacteria for RNA seq. I have used fresh samples for extraction as well as 3 days stored (-80) samples. After gel electrophoresis I didnt observed 3...

04 December 2018 8,059 10 View

What are the possible reasons to decrease in wear rate?

Dear All, What may be the possible reasons to decrease in mass loss and wear rate with rise in load and temperature successively at a constant speed. Thank you.

26 November 2018 1,189 6 View

How to purify two different proteins with very close molecular weights and pI values?

I am trying to purify extracellular enzyme from culture supernatant, for that reason I concentrated SN and performed ion exchange chromatography using manual method (NaCl gradient in buffer,...

15 November 2018 8,265 10 View

Is it possible to do aero acoustics analysis using Ansys Fluent?

How to do Aero acoustics analysis of the 2 D flow problem in Ansys fluent with k-epsilon model? What commands has to be given for this analysis in this commercial software?

31 May 2018 8,673 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View