Can anyone suggest me the optimal dose and time required for MG132 treatment in mouse embryonic fibroblast (MEF) cells?

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Han Zhang

Hi Zulfiker, the dosage and the duration of MG132 treatment depend on the cell type. I once treated B lymphoma cells with MG132, and here attached my paper for your reference.

Article Bifurcated BACH2 control coordinates mantle cell lymphoma su...

Hope it would help you!

Syed Mukhtar Ahmed

Hi Zulfiker, I highly recommend you determine this empirically. One way you could find the dose is by doing a dose response experiment and looking at beta-catenin stabilization in say a given amount of time. Then adjust the dose according depending on how long you need the treatment for your experiment.