How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to dissolve L-tryptophan in PBS?

The solubility of L-tryptophan is 11.4g/l in water. I tried dissolving 1.14 g in 100 ml PBS but still, it's not fully dissolved. Should I add NaOH or HCl? Also, can we stir the solution, or...

22 January 2021 1,127 3 View

Which form of tryptophan and tyrosine is highly soluble in water?

The solubility of tryptophan and tyrosine in water is low. Is there a very highly soluble structurally similar or derivatives of these amino acids I can use? it doesn't matter if it is...

20 December 2020 3,157 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

What protein about 100kDa is tyrosine phospholated when challenge T cell with anti CD3?

Hi, I uesd purified mouse CD3 T cell from spleen and stimulated these cells with anti mouse CD3 for different time. At least I can say two proteins are activated (black and red arrow). For the...

11 November 2020 7,202 3 View

Can anyone suggest a cause for a lack of fluorescent signal from a Diorex UltiMate 3000 RS detector in an HPLC experiment?

I am trying to detect viral capsids using an UltiMate HPLC system. I have a strong peak at the expected retention time using a 260/280nm UV detector, however I am not receiving any signal from the...

27 October 2020 3,786 2 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

Which procedure should I perform in an azo coupling reaction where histidine and tyrosine are used as the coupling agent?

I used L-Histidine and L-Tyrosine as coupling agent for azo coupling reaction and sodium hydroxide solution to adjust pH. Although color change was observed during the coupling, no precipitate...

26 May 2020 8,342 7 View

Can you please help me to choose a kit for amino acid analysis?

Hello, I have to quantitate free amino acid in my sample using LC-MS/MS. My sample mainly is an assay for checking the enzyme activity of phenylalanine hydroxylase in converting phenylalanine to...

23 April 2020 8,153 2 View

How does downregulation of T cell receptor and CD8 molecules work on a molecular level?

Hello everyone, I have a question about my research topic. I used a tyrosine kinase inhibitor (Dasatinib) to improve detection of CD8+ cells, according to a few publications (Wooldridge et al....

29 March 2020 7,961 2 View

No title

We quantify amino acids extracted from meat after acid hydrolysis, but with that method we lose tryptophan and cysteine. Is there a way to extract these amino acids to quantify them by...

02 March 2020 3,442 2 View