Hello Nutan

Please refer to the research article below. It describes methods for in vitro as well as cellular based assays for protein kinase inhibitors.

Article Measuring and interpreting the selectivity of protein kinase...

All the Best.

I recommend you to read the following paper:

Jariteh M, Ebrahimzadeh H, Niknam V, Mirmasoumi M and Vahdati K(2015) Developmental changes of protein, proline and some antioxidant enzymes activities in somatic and zygotic embryos of Persian walnut (Juglans regiaL.). Plant, Cell, Tissue and Organ Culture. 122:101–115.