How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

How to obtain thermodynamic potential differences of a reaction?

Adsorption reaction on SnO2 surface Say for ex. 2nh3 + 3O- NOx + 3h2o + 3e 2H2S + 15O- 2SO2 + 2h2o + 6e

02 August 2020 216 3 View

In Ag doped SnO2 nanoparticles, can we find silver ions on the surface? In a redox gas sensing using Ag doped SnO2, in what form silver attributes?

In a gas sensing process using Ag doped SnO2 nanoparticles on silicon substrate, in what form silver is present on the surface before adsorption and during adsorption of a target species. Can we...

15 June 2020 9,641 3 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

What is the substitute of soil in experimental purposes?

Soil as a reference is very complex and need to be simplified for experimental purposes. What could be a true substitute for this soil medium.

23 May 2017 381 5 View

What are the possible devices that could be used to study sleep in earthworms?

I need details to study sleep in earthworms belonging to different functional categories. The problems are: (i) alternative medium to replace soil, (ii) colour of light to be used, (iii) flexible...

16 October 2015 5,323 4 View

Is anyone familiar with the green disposal of both organic and inorganic wastes in bulk, of a Pharmaceutical Industry?

08 April 2015 1,556 3 View

How can a bio-sensor be designed and developed for detection of plant metabolites specifically flavonoids?

05 August 2014 3,094 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

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25 February 2021 5,713 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

How can we determine the band gap of a composite material that is made up of a composition of direct and indirect band gap semiconductor materials?

I have a direct band gap material and an indirect band gap material. If I made a composite of both through physical mixing. Then how can I determine the band gap of such a material as the Tauc...

17 February 2021 8,104 4 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

How can I get good bands by silver nitrate staining on SSCP method?

Hi Dear colleagues This was my first experience on SSCP, and I couldn`t get any band by silver staining! Staining with Et Br was successful. I mixed the PCR product with formamide loading dye,...

01 February 2021 8,788 6 View