Can anyone suggest me best protocol for Goat igG - HRP conjugation?

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Falk Fish

The classical periodate activation method can be located in many publications and methods books.

Vimarsh Gowda

@ Falk: thanks, as I am new to this stream, if you can recommended some link, it will be easy for me..

Tiziana Di Febo

I use the periodate method for the conjugation of monoclonal and polyclonal antibodies with HRP.

How good with glutaraldehyde method? Any comparative experience of betterness?

R.P. Achterberg

Hi, Take a look at www.innovabiosciences.com

Their Lightning-Link kits are great. 30sec handson time. Good conjugation

Here is a link

http://www.protocol-online.org/prot/Protocols/Peroxidase-Conjugation-by-Periodate-Method-440.html

This is the protocol that I use.

Great ... Thanks everyone.. Being a baby in protein science, this will be of great help. Thank you all...