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18 November 2020 9,422 1 View

What is the good method for cleaning tungsten fiber before coating process?

I am working on tungsten fiber coating. My first step is to remove oxide contaminants on the surface. Please suggest me some Pre-treatment methods.

19 May 2019 5,741 3 View

Can I know how to do sensitivity analysis using R for the the given data??

The 2 columns variables and values are the input to some design and I observed those remaining column values as output. Please tell me how to write R program to have sensitivity analysis for such...

08 November 2017 9,141 1 View

During Spray Deposition process, if a billet/substrate experiences stress higher than yield, does it mean that substrate material failed(not usable)?

Or is it okay for the substrate to experience such high thermal stress?

27 August 2017 6,892 2 View

How to solve non-positive volume cells mesh issue in icepak?

Exact message "Warning: The mesh contains non-positive volume cells.This must be fixed before solve." The model is made up of .igs CAD file import. It has curved surfaces. Is there a way to check...

30 July 2017 237 3 View

Is there any book recommeded for spectrum sensing methods?

06 May 2017 4,110 4 View

Any suggestions on a high background for an ELISA reading?

Dear Friends, From past many days, i am getting problems with very high background reading for ELISA. 100ng Antigen Coated Skimmed milk 5% coating buffer having 0.5% tween Rabbit serum dilutions...

20 May 2015 9,179 13 View

What is the best method for cloning long PCR fragments?

We are trying to generate an infectious clone of a plant virus called cassava brown streak virus in 3 fragments of 3-4 Kb in size (virus is ~10Kb) but have been failing to get more than one clone...

10 October 2013 9,883 15 View

Minimum Uncertainity of Gaussian Wavepacket?

Why does gaussian wavepacket have a minimum uncertainty product? Is it because of the way variance is defined?

20 April 2013 6,362 6 View

How to evaluate the integral 1/(a1*x^n + a2*x^(n-1) + ... + a(n-1)*x + an)?

When n = 2 or 3, we can solve this integral by reducing the denominator to the form (x + a)^2 + B. What is the general procedure for evaluating such integrals for n > 3?

05 March 2013 7,657 8 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View