Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

23 June 2015 3,340 3 View

Can someone give me a suggestion on the cloning procedures?

11 March 2015 1,728 4 View

Does anyone have experience with, or literature on, bone plow or rake phenomenon using locking plate fixation ?

When bone is cut by locking screws. In my experience in metaphyseal bone.

08 February 2015 5,366 2 View

Who knows sites hosting free samples of images with known clusters inside to test clustering machine learning models?

I have several clustering models to be tested and validated on images for which clusters inside are well known.

23 March 2014 7,277 2 View

Could somebody please give me the nucleotide sequence of the TEV protease recognition site?

I would like to use this sequence for TAP of protein complexes.

17 February 2014 7,498 7 View

Which activities should be the most important for academic people?

Normally an academic dedicates do some hours per week to research, some to teach and some to administration of the Department or Faculty. Some hours are also dedicated to external work, as...

01 November 2013 4,783 3 View

Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

I am trying to identify histone locus bodies in a prostate cancer cell line (PC-3)

09 July 2013 280 3 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

20 June 2013 1,849 19 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

05 April 2013 5,771 10 View

Are the telomere length different in human skeletal muscle upper limbs respect lower limbs?

Is there any evidence that telomere length in human skeletal muscles is limb specific?

03 April 2013 8,558 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View