Multiplex qPCR annealing and extension?

Dear All, I have set up a 5-plex qPCR. The multiplex works fine but the PCR efficiency doesn't look that great. I am throwing in 250ng human genomic DNA and I get Ct in range of 30-35 for all my...

13 February 2020 7,095 3 View

Is it feasible to use RNN model for improving the visual quality of the image(image enhancement)?? I am unable to find any paper related to this?

I am working on image processing especially in the area of image enhancement. But I am unable to find any paper related to RNN model for improving the quality of the image.

11 February 2020 140 2 View

RNA extraction from MTB?

Dear All, I am looking for a kit to extract total RNA from MTB which I will later use for RT-qPCR. My initial sample would be very low and cannot afford to loose RNA during the extraction. Also,...

09 July 2019 1,278 5 View

How to store root tissue for a week which is already treated with DAF-FM DA for NO fluorescence estimation as microscope is not available?

The root tissue is already incubated in the DAF-FM DA and washed with HEPES-KOH buffer. But due to non-availability of confocal microscope on the same day, is it possible to store the tissue in...

26 July 2018 847 2 View

What tests can be used for descriptive research?

Hi, will appreciate expert opinion on the below: 1. Can 'impacting' or 'relationship' hypothesis be formulated for descriptive research? 2. Can correlation, regression and chi-square tests be...

27 March 2018 4,858 7 View

Continous column adsorption capacity

If i am working on a column filled with adsorbent, in my batch study max adsorption capacity was achieved in 1 hr. In continous column how can i achieve that max adsorption capacity that was...

26 June 2017 4,191 7 View

In my model of matlab for fixed bed adsorption column, i have obtained very large time in X-axis of breakthrough curve?

In my model of matlab for fixed bed adsorption column, i have obtained very large time in X-axis of breakthrough curve..i.e nearly 5*10^(5) second. Is it possible? why it is higher. please suggest?

03 May 2017 4,295 3 View

What solvent system i should use for running a TLC for water soluble compound ?

i have a bacterial crude extract soluble only in water .........what solvent system i should use to run TLC or column

10 February 2017 499 4 View

Positive delta G?

07 January 2017 5,042 3 View

Can pikovskaya isolated bacterial colonies be transferred on nutrient agar plate ?

I have isolated bacterial colonies on pikovskaya agar plate but when transferring them to nutrient agar plate as well as nutrient broth for purification they were not growing

26 April 2016 9,360 3 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View