Hi H.

On the internet you could find a lot of firms/manufacturers, who sell  IL-18 ELISA kits for mouse/rat/human

some examples attached

http://www.rndsystems.com/product_results.aspx?r=0&c=56&s=0&a=0&m=0&k=IL18&b=False&cj=-1&o=AND&p=0

http://www.abnova.com/products/products_detail.asp?catalog_id=KA0561

http://www.abcam.com/products?keywords=IL18&selected.classification=Immunoassays+kits+and+reagents

Thank you sir...

But you have original protocol of IL-18 research article.

Hi H.Ram

in our department we work with the luminex system for this kind of analysis

so i dont have a protocol for elisa work.