I want to check the effect of arsenic on some plant genes expressed in Saccharomyces for which i need a strain tolerant to arsenic treatment.
Can anyone suggest an easily available strain for my work?
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Recently, the Ministry of Agricultural and Rural Affairs enacted the approval of twelve genetically modified (GM) soybean varieties, marking a strategic pivot in China's quest for food security....
05 June 2024 1,143 0 View
A plasmid of mine has GOI in opposite orientation. I have another plasmid which has exact same RE site but in such a way that it orients my GOI in right direction. However even after multiple...
05 May 2024 5,662 2 View
Thank you for reading my question. Currently, I am doing parameter estimation of Lithium-ion battery by using RLSF Algorithm. I am getting all the parameters as negative value. Which is not...
17 April 2024 8,941 0 View
I am planning to study the overexpressed pattern of lncRNA. Could you explain whether the primer designing procedure for cloning a long non-coding RNA (lncRNA) gene follows the same approach as...
14 April 2024 3,966 3 View
Please recommend any organic solvent for dissolving mucilage.
30 January 2024 5,692 5 View
Which process or technique will work? Will LC-MS give me a full enzyme profile?
26 January 2024 3,374 1 View
Does it make a difference whether I conduct drug treatment in a 6-well plate, utilizing multiple wells and subsequently pooling the samples to achieve one million cells, or if I should opt for...
21 January 2024 9,405 0 View
I am interested in knowing if there is any sgRNA library designed against MDCK cell line which is a canine cell line. i believe it shares about 84% homology with human cell line. let me know if...
03 January 2024 5,148 0 View
My paddy seeds are not germinating properly. I have tried different methods to break dormancy like- hydropriming, Dry heat (45 degrees for 2 days), and acid treatment (1.5% HNO3) at 30-32 degree...
01 January 2024 7,000 6 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...
18 June 2024 2,106 0 View
Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...
17 June 2024 1,069 3 View
I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...
10 June 2024 5,941 3 View
I am interested in the safety/toxicity of NTBC administration in different animals. There is a lot of data available relating to oral administration however limited data on intravenous administration.
04 June 2024 5,628 1 View
for ABO3 if only A & B then we used t=rA+Ro/sqrt2(rB+rO) kindly explain for BaSrCaTiO3 ?
31 May 2024 2,036 0 View
Is the instant dry yeast, or Brewer's yeast, Saccharomyces cerevisiae, had the ability to provide calcium and nitrogen minerals in addition to ethahnol ? Please support answers with published...
28 May 2024 1,073 4 View
I want to find basin of attraction for delayed logistic map like this: x(n+1) = A*x*(1-x(n)-B*x(n-1) I wrote bellow (MATLAB) code, but it just show three basin of attraction for given parameter...
21 May 2024 1,686 0 View
I am checking GFP fluorescence level in heme sensor. 200 ul of sample was loaded in black flat bottom 96 well plate in H1 Synergy machine. Temperature was 30 degree. I am using S. cerevisiae WT...
06 May 2024 5,744 3 View