If you are doing ELISA and PCR then use Qiagen.

A Qiagen DNeasy Blood and Tissue Kit.

Qiagen ,is the best

Thank you Drs. Iqbal, Pimsler and Ramaiya.

You can find a recent review on this topic here:

Comparison of manual and semi-automatic DNA extraction protocols for the barcoding characterization of hematophagous louse flies (Diptera: Hippoboscidae).

Rafael Gutiérrez-López, Josué Martínez-de la Puente, Laura Gangoso, Ramón C. Soriguer, and Jordi Figuerola.

Vol. 40, no. 1 Journal of Vector Ecology 1

Estación Biológica de Doñana (EBD-CSIC), C/Américo Vespucio, s/n, E-41092, Seville, Spain, [email protected]

Received 13 May 2014; Accepted 16 September 2014

ABSTRACT: The barcoding of life initiative provides a universal molecular tool to distinguish animal species based on the amplification and sequencing of a fragment of the subunit 1 of the cytochrome oxidase (COI) gene. Obtaining good quality DNA for barcoding purposes is a limiting factor, especially in studies conducted on small-sized samples or those requiring the maintenance of the organism as a voucher. In this study, we compared the number of positive amplifications and the quality of the sequences obtained using DNA extraction methods that also differ in their economic costs and time requirements and we applied them for the genetic characterization of louse flies. Four DNA extraction methods were studied: chloroform/isoamyl alcohol, HotShot procedure, Qiagen DNeasy® Tissue and Blood Kit and DNA Kit Maxwell® 16LEV. All the louse flies were morphologically identified as Ornithophila gestroi and a single COI-based haplotype was identified. The number of positive amplifications did not differ significantly among DNA extraction procedures. However, the quality of the sequences was significantly lower for the case of the chloroform/isoamyl alcohol procedure with respect to the rest of methods tested here. These results may be useful for the genetic characterization of louse flies, leaving most of the remaining insect as a voucher. Journal of Vector Ecology 40 (1): xxx-xxx. 2015.

Keyword Index: DNA extraction methods, Barcoding of life, COI, Hippoboscidae, Ornithophila gestroi, parasites.

I am now out my office but in a few days I will try to get for you a noncopyrigth version. I hope to have available definitive one very soon.

Additional information:

1.- Be carefull about the amplification process. Previously, check that you amplify only the DNA target . So, be carefull when you use standard primers in new mosquitos species (never tested before). I.e. we got  this problem with Aedes using standard primers. To resolve the problem we develop new protocole for this species.

2.- One of the main problem you have in this kind of studies it is the quality of the DNA. So, the extraction and amplification process are critical for getting a clear sequence. The quality of the sequence it is the base of the a robust identification of the host species.

In my previous reply I sent a abstract about our experience on this topic. This experience came from longterm studies of feeding mosquitos from Russia, Senegal, Italy, Austria , Israel and Spain. Depending of the sample size, can be more expense (until 3-4 time more) to use some protocoles (i.e Qiagen..) instead of the other one. More information about that can be found in our papers availables from our profile in Researchgate: https://www.researchgate.net/profile/Ramon_Soriguer

Do not hesitate if you need more information about this topic

Qiagen blood and tissue kit will not do what i asked. I am interested in carrying out blood meal origin ELISA. I want to detect the source (human blood, bovine, e.t.c) of the blood in the fed mosquito samples collected in the field and i am talking about getting a single supplier for the reagents. I have tried KPL but they do not have lyophilized form of  human, chicken, goat and bovine sera.      

I am sorry. I understood you wrongly

However, for the great work that requires getting fed female mosquitoes samples I recommend studying feeds via amplification of DNA. My colleagues from the Pasteur of Senegal and CRIE of Russia began their feeding studies with ELISA and currently have stopped. They use the same technique to us. At present we are using it with Culicoides and black flies.

Sorry I can not help using ELISA. I have no experience.

Good luck

Hi colleague,

I don’t know if their is any single source/supplier of all the reagents for blood meal or if this will be helpful but you can try the ffollowing link:

http://www.rockland-inc.com/Default.aspx

Good luck,

Dr El Hadji Amadou Niang

Laboratoire d’Écologie Vectorielle et Parasitaire

UNIVERSITÉ CHEIKH ANTA DIOP DE DAKAR

Thanks Dr. Niang,

they don't have all the capture and conjugated mabs.  

Sorry this is coming late, I got the capture and conjugated mabs from KPL and the sera from rockland immunochemicals. It turned out to be perfect.