Dear Selvakumari Ulagesan,
The following publication describes a good method for quinoline isolation from plants:
Rapid and Green Analytical Method for the Determination of Quinoline Alkaloids from Cinchona succirubra Based on Microwave-Integrated Extraction and Leaching (MIEL) Prior to High Performance Liquid Chromatography
Anne-Sylvie Fabiano-Tixier 1, *, Abdelhakim Elomri 2 , Axelle Blanckaert 2 , Elisabeth Seguin 2
,
Emmanuel Petitcolas 1 and Farid Chemat 1
1 UMR 408 INRA, Safety and Quality of Plant Products, University of Avignon, 84000 Avignon,
France; E-Mails: [email protected] (E.P.); [email protected] (F.C.)
2 CNRS UMR 6014, C.O.B.R.A., UFR Medicine-Pharmacy, University of Rouen, 76183 Rouen,
France; E-Mails: [email protected] (A.E.); [email protected] (A.B.);
[email protected] (E.S.)
* Author to whom correspondence should be addressed; E-Mail: [email protected];
Tel.: +33-4-90-14-44-26; Fax: +33-4-90-14-44-41.
Received: 9 September 2011; in revised form: 21 October 2011 / Accepted: 31 October 2011 /
Published: 14 November 2011
Abstract: Quinas contains several compounds, such as quinoline alkaloids, principally quinine, quinidine, cinchonine and cichonidine. Identified from barks of Cinchona, quinine is still commonly used to treat human malaria. Microwave-Integrated Extraction and Leaching (MIEL) is proposed for the extraction of quinoline alkaloids from bark of Cinchona succirubra. The process is performed in four steps, which ensures complete, rapid and accurate extraction of the samples. Optimal conditions for extraction were obtained using a response surface methodology reached from a central composite design. The MIEL extraction has been compared with a conventional technique soxhlet extraction.
The extracts of quinoline alkaloids from C. succirubra obtained by these two different methods were compared by HPLC. The extracts obtained by MIEL in 32 min were quantitatively (yield) and qualitatively (quinine, quinidine, cinchonine, cinchonidine) similar to those obtained by conventional Soxhlet extraction in 3 hours. MIEL is a green technology that serves as a good alternative for the extraction of Cinchona alkaloids.
2. Experimental Section
2.1. Apparatus
The basic principle of the process of microwave-integrated extraction and leaching is illustrated in the Figure 1. MIEL extraction has been performed in Milestone NEOS microwave oven. This is a multimode microwave reactor 2.45 GHz with a maximum delivered power of 1000 W variable in 10 W increments. Temperature was monitored by an external infrared (IR) sensor. During experiments, time,
temperature, pressure and power can be controlled.
For more details on the method, please use the following link:
https://www.google.ps/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwiIz7r3uqjMAhVrBMAKHUwYBXQQIAghMAA&url=http%3A%2F%2Fwebcache.googleusercontent.com%2Fsearch%3Fq%3Dcache%3ArymA862sMYIJ%3Awww.mdpi.com%2F1422-0067%2F12%2F11%2F7846%2Fpdf%2B%26cd%3D1%26hl%3Den%26ct%3Dclnk%26gl%3Dps&usg=AFQjCNFvojKiEGha6NcBwZPX8FGW2Sk9hg&sig2=R7h-VFjS_P0vtGnfZF9T6Q&bvm=bv.119745492,d.ZGg
Hoping this will be helpful,
Rafik
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