Most fungi sporulate well on oatmeal agar and cornmeal agar.

Another old discussion here might be useful in this regard: https://www.researchgate.net/post/Could_somebody_suggest_a_procedure_for_favoring_the_development_of_spores_rather_than_that_of_hyphae

Other than the papers cited in the above discussion you can also have a look at this article where they specifically mention oatmeal agar to be best for Fusarium spp.

https://www.researchgate.net/publication/228489602_Growth_Characteristics_of_Fusarium_Spp._Causing_Wilt_Disease_in_Psidium_Guajava_L._in_India?ev=prf_pub

All the best.

Article Growth Characteristics of Fusarium Spp. Causing Wilt Disease...

There are mediums for fusarium oxysporum that produce good sporulation. You can find them in literature. WA with soil is good medium to produce very good sporulation. if like i could send to you list of mediums. Best regards

Most fusaria sporulate well on tap water agar with a few sterile rice grains placed on the agar. I usually use 4 to a plate. You can place the plate on a microscope stage to check for conidial production. Inoculate the culture in the center of the plate and let the fungus grow from there.

potato dextrose agar but the traditional preparation with potatoes water and agar, the fusarium sporulate better than in the comercial potato dextrose(PDA)

I also commonly use PDA and SNA medium.

I sporulate all my fusaria species on PSA at pH6.5 setted with CaCO3

Per liter you have: 200 g of Potatoes extract, 20 g of Sucrose (saccharose) and 20 g of Agar. It works well.

Good luck

Most fungi sporulate well on oatmeal agar and cornmeal agar.

Another old discussion here might be useful in this regard: https://www.researchgate.net/post/Could_somebody_suggest_a_procedure_for_favoring_the_development_of_spores_rather_than_that_of_hyphae

Other than the papers cited in the above discussion you can also have a look at this article where they specifically mention oatmeal agar to be best for Fusarium spp.

https://www.researchgate.net/publication/228489602_Growth_Characteristics_of_Fusarium_Spp._Causing_Wilt_Disease_in_Psidium_Guajava_L._in_India?ev=prf_pub

All the best.

Article Growth Characteristics of Fusarium Spp. Causing Wilt Disease...

Could try on SDA or PDA 5-7 days at 30C

I find that Fusaria and many other filamentous fungi sporulate more quickly if the medium is more dilute than the manufacturer's suggestion, half-strength or even less. This is particularly true for endophytic fungi, which can be osmotically shocked by transfer from the plant to a full-strength medium.

In link The_Fusarium_Laboratory_Manual_

Best wishes

https://www.facebook.com/groups/drkhaledarafat/

I agree with several of you PDA has provided the best and most rapid growth at our facility for this type culture

Quarter PDA is a good media that enhances sporelation

Carnation leaf agar is the best medium for spore productionbut i saw Mungbean agar also as a medium for spore production but we try to do it unfortunatly this last medium did not response

I do agree with Dr. Sayed Sabbagh using carnation leaf agar for production of Fusarium conidia...

PDA is best

To have a good production of fusarium conidia, you must have a strain recently isolated. It depend on the strain that you use.

To stimulate the sporulation, you can incubate PDA Petri dishes at room temperature in a bright room. The UV enhance the production of spores .

vous pouvez utiliser l'eau gélosée à 2% en boite : 20 g agar dans 1000 ml eau distillée. C'est un excellent milieu pour stimuler la sporulation.

Thank you for your suggestions

its simple as like and prepare a water agar with pieces of sterilized filter papers placed on the top, inoculate Fusarium on the filter paper, you get sporulation .. try this

Dear Riddhi,

it's very easy: I use PDA (potato dextrose agar), in Petri disches, 26-28°C, under natural or artificial light.

Dear Riddhi

The cheapest effective method is to prepare a medium with distilled water plus wheat stem fragments in flasks and inoculate the medium and incubate it under artificial lamp at room temperature. A method similar to that suggested by dear Gunasingham but with wheat stem fragments and water in a flask.

F. oxysporum is an easy fungus to sporulate, use corn meal, oat meal , PD medium but do not add sugar . you might have black light which is far UV light , If so incubate your culture under such light for a week at a 20 degree celsius, you will get a massive spores ,If not the regular incubator is good enough

go for cellophane technique with exposure to light 16hrs of light. Do side culture and observe.

Dear Riddhi,

we induce sporulation of F. oxysporum (a lot of f. sp.) on PDA, oatmeal, and cornmeal agar. With or without lighth

Use oatmeal agar and temperature 5 -25°C

If you use UV to induce the spore, what is the wavelength for the UV?

Dear Riddhi, I have used pieces of carnation leaves after irradiation to produce conidia in stead of hyphae...

2% water agar would also do the trick. I guess it also depends on the virulence of your strain.

I have used PDA, corn meal,oatmeal, Sabouraud ,however some species of Fusarium have had good sporulations, but other didnt have same behavior, I recommended you try with changes light and temperature, for example exposure them to light 12 or 16hrs of light, at 5 to 25 degree celsius.

I had used Moong bean agar and it was good for sporulation.

The easiest way is to just use PDA and then scrape the surface for spores. That should be sufficient for most F. oxysporum isolates. In case you are working with a low sporulaton isolate, try cultivating it in liquid Fusarium Complete Medium (CM) at room temperature, low agitation and dark conditions for 5-7 days. You can then collect the spores by filtering through a double layer of cheesecloth and centrifuging to collect the spores.

Best of luck

You can use OA and CLA media,

Good luck

Fungi in general, and Fusaria in particular, the environmental conditions necessary for good sporulation are more limiting than are those growth. A number of microbiological agar media such as Malt Extract Agar, Oat Meal Agar, etc. are useful for Fusarium sporulation. However, Potato-dextrose Agar is more efficient in the sporulation of Fusarium. Other natural media such as cereal straws, seeds embedded in water agar, green carnation leaves, and Nash medium are excellent for asexual sporulation in Fusarium.

I think use PDA with additional supplements. like 1 gm of glucose, peptone, yeast extract. Add nitrogen supplement in the media too. Like ammonium tartrate (0.5 M 5-to 10 ml /L of media) and maintain the pH of the media upto 6.5. I hope that will enhance the sporulation. Best wishes

I get sufficient sporulation using Potato Dextrose Agar.

Rose bengal medium along with ample of light to culture will be able to give more sporulation.

Very easy!!! use PDA at 1/2 or 1/4 of the normal concentration (add  1 or 2% agar to solidify the medium)

Rose Bengal agar and PDA is good for growth

You can also use CLA (carnation leaf agar)...is highly good to produce Fusarium microconidia

I suggest that PDA medium for quick growth of fungal culture and sporulation also.

CMC (Carboxymethylcellulose) liquid media works wonders for sporulation. YPD for tissue. 

Hello Riddhi

Maximum mycelial growth was obtained in potato dextrose agar as semi-solid while Maximum sporulation will obtained in oatmeal agar and mycological broth. Please see the attached references

Good luck

What about the wheat blast fungi sporulation? How can the rapid sporulation of wheat blast fungi be induced? Is there any other way other than using different culture medium?

have a look. it will help u.

PDA+YPD is the best media

Carnation leaf agar works perfectly.