Can anyone suggest a good/working Anti-Biotin antibody to be used in a human cell line?

More Ashik Jawahar Deen's questions See All

How to dissolve photoinitiators TPO and Irgacure 2959 for cytotoxicity assay?

Hi. I am working on a project to study the cytotoxicity effects of various photoinitiators on different cell lines. However, I am not sure how I can dissolve Irgacure 2959 and TPO since using...

26 October 2023 8,279 2 View

How to get my research back?

How can I claim my paper? Someone use my name and research paper .

02 August 2023 902 1 View

Can I compare infraclass-correlation coefficient (ICC) found from two models?

I have calculated two cross-classified multilevel models where the models have different sample sizes. I was wondering whether I can compare (like say higher or lower) ICC between these two...

25 May 2023 1,654 2 View

Can we use celestite as a substitute for barite to improve bentonite as drilling mud for oil wells?

Iraq has no barite, but celestite can be found on some sites, need to use this celestite as a partial or total substitute for barite in thinking the drilling mud in the oil industry in Iraq

15 April 2023 565 0 View

Is precipitated calcium carbonate is nano calcium carbonate?

we have a process to precipitate CaCO3 we need to control product particle size

11 March 2023 4,920 7 View

List some hot topic of research on Brain Computer Interface in 2023 for MSc student of CSE?

Reserch will include Machine learning, deep learning based custom model. Suggetion on device with minimal cost for capturing brain signal will help additionaly.

19 January 2023 5,121 4 View

Is Creep-Resistant Aluminium Alloy different from Wrought Aluminium Alloy?

I have the concept of Creep-Resistant Aluminium Alloy in my PG academic subject. I need someone to clarify whether Creep-Resistant Aluminium Alloy differs from Wrought Aluminium Alloy.

09 December 2022 4,512 5 View

Doubts on Radiator/Compact Heat Exchanger Sizing!?

I have calculated the power losses of the system and the inlet and outlet temperatures for both air and waterside. Using LMTD method, the overall heat transfer coefficient of the CHE was found. I...

19 October 2022 3,639 1 View

Is there a software for designing and simulating Compact Heat Exchanger?

I am trying to size a radiator for a car. Is there is a specific software used by automobile engineers for sizing the radiator? Can I use Aspen EDR for compact heat exchangers?

17 October 2022 1,478 2 View

My research team need secondary experimental data on the behaviour of tall buildings under wind loads; unprocessed data. Can someone please help?

Hi All I am participating in research on numerical simulation of tall buildings' behaviour under different levels of wind speeds (in terms of magnitude). My team need secondary experimental data...

14 September 2022 8,040 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Markus Sack

I have done experiments with anti-biotin mAbs from Miltenyi some years ago and the nice feature was that they only recognized the conjugated biotin and not any excess of free biotin. Depending on the application that might be of advantage. Ofcourse you may also think about steptavidin / neutravidin conjugates for staining cells...

Ashik Jawahar Deen

Thanks a lot for your suggestion! I need anti-biotin antibody for an antibody-based assay (PLA) and so streptavidin/neutravidin conjugates may not help in this case. I will look into your suggested antibody from Miltenyi! Thanks again!