I am willing to use crispr cas9 technique for gene knockdown in HT29 cell line for my research. Can anyone suggest a good and reliable protocol or kit for the same?
Hi there
Please check Feng Zhang's web page. It is full of tips and tricks about how to use CRISPR system in general purpose applications.
http://www.genome-engineering.org/crispr/
Hope it helps. Best
Paco
I have successfully used following protocol for knockout of multiple genes (although not in HT29).
Article Genome engineering using the CRISPR-Cas9 system
You can check out Applied Biological Materials Inc. (www.abmgood.com). They have CRISPR-KO generation services.
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