Can anyone suggest a good protocol or kit for crispr cas9 mediated gene knockout/knockdown in animal cell culture, HT29 cells?

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Francisco J. Enguita

Hi there

Please check Feng Zhang's web page. It is full of tips and tricks about how to use CRISPR system in general purpose applications.

http://www.genome-engineering.org/crispr/

Hope it helps. Best

Paco

Kashif Aziz Khan

I have successfully used following protocol for knockout of multiple genes (although not in HT29).

Article Genome engineering using the CRISPR-Cas9 system

Leo Urmaza

You can check out Applied Biological Materials Inc. (www.abmgood.com). They have CRISPR-KO generation services.