I have an mCherry signal in my zebrafish and would like to detect it with antibody, which will enhance the signal. Does anyone have any recommendations?
Also - can I use a mouse anti-mCherry for detection in zebrafish?
Thank you!
Monika - For your second question, you can use mouse anti-mCherry for detection of mCherry from any species. The antibody was made from mice, but the epitope is present on all mCherry proteins.
I used Abcam anti-DsRED with success. mCherry is a derivative of his. It gives a strong signal with low to no aspecific signal.
Hi Monika, in our zebrafish lab we use a mouse anti-mCherry antibody that works very well in our hands. It is made by Abcam, code ab125096. Cheers, Natascia
Hi Monica,
I use rabbit anti-RFP from antibodies-online (ABIN129578) at 1:3'000. Works quite nicely.
Best, Thomas
check OriGene's anti-Cherry mAb
http://www.origene.com/antibody/AntitagAntibody.aspx?tag=mCherry
Can anyone suggest a good mCherry antibody for formalin-fixed and paraffin-embedded tissues?
Ideally, it should not be a mouse on as it is needed for reporter detection in mouse tissues.
Thanks in advance
Hi,
If anyone is still looking for a reliable anti-mCherry antibody, you might want to try the guinea pig anti-rfp from Synaptic System (#390 004). It works perfectly on PFA-fixed mouse brain tissue (infected with AAV-Dreadds-mcherry)
Cheers,
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