What would be the best marker to label ALL (or as many as possible) glia cells in zebrafish?

I am identifying cells that express my gene of interest and I think they might be glia cells. I would therefore need a reliable marker that would label as many glia as possible - Muller,...

05 June 2017 7,937 1 View

Zebrafish mounting for confocal microscopy?

11 December 2016 1,494 6 View

Would you usually clean up linearized plasmid before using it for ISH probe?

I am creating an in situ hybridization probe and am linearizing 10ug of plasmid DNA containing my construct. Would you clean up the product before using it for T7 transcription or would you use it...

09 October 2016 9,976 6 View

Long range PCR optimisation?

07 August 2016 2,098 9 View

Up-to-date CRISPR knock-out protocol in zebrafish?

07 August 2016 2,347 6 View

Evaluating Tol2 F0 results in zebrafish?

I am performing transgenesis in zebafish, using a Tol2 system. I have successfully constructed the entry plasmid and the integration of my wished construct has also been confirmed. However, I do...

03 April 2016 8,648 9 View

What do I do when my RNA purity is over 3?

02 March 2015 308 14 View

How do I know where will the Tol2 transposase cut my plasmid when injected into zebrafish?

I have a final Gateway Tol2 construct ready for microinjection into zebrafish embryos. I also have a pCS-zT2TP zebrafish transposase mRNA, which I will co-inject with the construct. The question...

10 November 2014 2,277 2 View

Can anyone suggest a good anti-mCherry antibody?

10 November 2014 7,500 7 View

Any advise on the Gateway Tol2 Constructs?

I'm performing Tol2 enhancer and promoter assay in Zebrafish and would need a help with the constructs. I've ordered few of them from Addgene and they are on their way (for promoter assay: p5E-MCS...

08 September 2014 2,205 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View