I would try to test for long-term uptake of a fluorescent dye into cells and ex vivo tissue fragments.
Hi Amilcare, in addition to Calcein, here is a bunch of them:
http://www.lifetechnologies.com/search/global/searchAction.action?query=live+cell+membrane&resultPage=1&resultsPerPage=15&autocomplete=
But you might want to look into live cell dyes that can be fixed intracellularly by formaldehyde:
http://www.lifetechnologies.com/order/catalog/product/C2110?ICID=search-product
Hi Amilcare,
I think you can try Mitoracker red cmxros dye.
Best wishes
Susheel
What would you like to detect more specificially in the cell? Or only to introduce it within the cell??
DAPI and IP
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