Can anyone suggest a fluorescent vital dye suitable for detection up to 5-7 days in living cells/tissues?

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Steingrimur Stefansson

Hi Amilcare, in addition to Calcein, here is a bunch of them:

http://www.lifetechnologies.com/search/global/searchAction.action?query=live+cell+membrane&resultPage=1&resultsPerPage=15&autocomplete=

But you might want to look into live cell dyes that can be fixed intracellularly by formaldehyde:

http://www.lifetechnologies.com/order/catalog/product/C2110?ICID=search-product

Susheel Kumar Singh

Hi Amilcare,

I think you can try Mitoracker red cmxros dye.

Best wishes

Susheel

Adela Nano

What would you like to detect more specificially in the cell? Or only to introduce it within the cell??

Tomasz Cłapa

DAPI and IP