kindly let me know if it should be prepared in DEPC water or normal sterile water.
here it goes: RNA Denature buffer: 600uL formamide;210uL formaldehyde(37%);120uL 10xMOPs, 10mg/ml-Et.Br. Agorase gel should be prepared in DEPEC water.
-sk
I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.
18 January 2021 4,756 2 View
Is there any available data that shows reinfection by COVID-19 Virus? What is the immune status against COVID-19 virus of an affected person, once he/she gets cured?
01 June 2020 190 12 View
Minimally invasive surgery technique has taken the central role in modern surgery specially in abdominal surgery. So, every surgeons need to get trained into the field of minimally invasive...
25 May 2020 6,412 9 View
Hi everyone, I am looking for any recommendation on concentrating very low molecular weight proteins (~1-3 kD). I would appreciate if anyone has experience in dealing with that low protein size....
10 September 2019 3,853 4 View
I am modelling a uniacial compression test of cylinder in abaqus. I need to extract principal stress value.I extracted force on surface, divided by area to get engineering stress value. After...
17 June 2018 5,843 14 View
Kindly suggest me some tips or precautions to avoid fungal contamination in Drosophila culture tubes. In my case contamination persists even after flipping of flies, and contamination seriously...
02 April 2015 7,818 9 View
Want to know in a nutshell; what information can be & can not be obtained by each of the whole genome molecular analysis approaches. Any book / paper / answer please? Thanks!
01 January 1970 4,364 3 View
The recent paper published in Nature Geo-science by Goodwin and associates talk over that if immediate action (measures to control GB) isn't taken, the earth's global average temperature is likely...
01 January 1970 2,422 17 View
Have noticed that several events are being linked to climate changes including global warming but scientifically they are not. Do have precise knowledge about the events which can be connected to...
01 January 1970 9,224 13 View
Water resources are the significant part of the ecological cycle which empowers the survival of all living beings on the earth system. It plays a major role in the development of the agricultural...
01 January 1970 9,292 16 View
Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...
14 February 2021 6,247 3 View
I want to incorporate CNT in the alginate solution
23 December 2020 9,521 3 View
In microbiology to do plating, is there any need to sterile the DI water also?
22 December 2020 3,288 1 View
As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...
22 December 2020 8,196 5 View
Hi, I want to start my cell culture to run a MTT assay , how can I sterilize my polymer?
15 December 2020 1,813 1 View
I have chitosan, silver nitrate and NaOH suspension, Paper says wash the suspension twice with distilled water and then centrifugre. How do I was a liquid. Sterile filter it ??? Any suggestions
14 November 2020 7,112 4 View
Powder insoluble
08 November 2020 509 3 View
I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...
04 November 2020 9,427 6 View
I left tubes with isolated DNA drying (before resuspension) in a biosafety hood for too long and a colleague came and turned on the UV light for some 20-30min. The light did not shine on the DNA...
29 September 2020 536 3 View
The reason for sterilization through heat sterilization in Beta-Galactosidase production process is to remove other bacteria from the air. If this process is designed, the bacteria that produce...
08 September 2020 8,120 1 View