I'm currently doing a research proposal on the effect of a certain herbal plant decoction. I need to do chemical fingerprinting of the said decoction. I hope you can help me with this.
Dear Genevieve,
You may use the following paper as start point for your research:
Zhong Yao Cai. 2012 Jul;35(7):1056-61.
[Study on HPLC fingerprint chromatograms of cell wall-broken decoction pieces of Notoginseng].
[Article in Chinese]
Chen WX1, Liu M, Yan P, Zhan RT, Chen WW, Cheng JL.
Author information
Abstract
OBJECTIVE:
To establish the HPLC fingerprint chromatograms of crude Notoginseng, cell wall-broken powder and cell wall-broken decoction pieces of Notoginseng and provide evidence for quality control of cell wall-broken decoction pieces of Notoginseng.
METHODS:
The HPLC procedure was performed on the chromatographic column of Hypersil ODS2, and the mobile phase was acetonitrile and water in gradient elution with the flow velocity of 1.0 mL/min. The detection wavelength was 203 nm and the column temperature was 25 degrees C. The chromatograms was analyzed with the software of "similarity evaluation system for chromatographic fingerprint of TCM".
RESULTS:
Eight common peaks were pinpointed from the chromatograms of different batches of crude Notoginseng, cell-broken powder and cell-broken decoction pieces, the similarities of the chromatograms were all larger than 0.9.
CONCLUSION:
The method of the HPLC fingerprint chromatogram is of good precision, reproducibility and stability,which is suitable for quality control of cell wall-broken decoction pieces of Notoginseng.
http://www.ncbi.nlm.nih.gov/pubmed/23252266
Hoping this will be helpful,
Rafik
Dear Genevieve,
It would be helpful to know the decoction method/concentration.Whether the decoction is of wet drug or dried drug.Marker compound will be of prime importance in the analysis.For standardization of natural product drugs, single chemical entities, “marker compounds,” may be used as potency standards in high performance liquid chromatography (HPLC) analysis. Using well-characterized marker compounds, conventional pharmaceutical manufacturing criteria for assay and content uniformity may be applied. These marker compounds may be used to help identify herbal materials, set specifications for raw materials, standardize botanical preparations during all aspects of manufacturing processes, and obtain stability profiles. HPLC analysis for marker compounds may provide additional information in the form of “chromatographic fingerprints.”
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