Hey every one,
I am staining Dorsal root ganglion neurons with beta 3 tubulin antibody but I have autofluroscence problem with the background and I should do tracing for neurites of neurons and the program traced also these noises in the background and the results are not accurate in this case . Also ,I used a confocal microscope to take photos. So please, if someone has any suggestion to overcome this problem, I will be so thankful.