Amira Elfarnawany

5 Questions 7 Answers 0 Followers

Questions related from Amira Elfarnawany

Why proipidium iodide (PI) did not detect cells with nuclear fragmentation(dead cells) ?

Hi everyone, I used propidium iodide as nuclear staining With concentration (5 µg/ml) for DRG nonneuronal cells for 2 hours pre-fixation to detect dead cells. I also used DAPI for staining all...

05 January 2023 5,336 2 View

What is the reason for these unspecific binding signals for 2ndry AB in photos?

Hi everyone, I did immuno fluroscence staining for non-neuronal cells but there are some unspecific signals in photos and spread in all photos like bright spots. Unfortunately, I donot know what...

25 July 2022 8,267 0 View

Can anyone recommend the best way to remove/reduce background Autofluorescence?

Hey every one, I am staining Dorsal root ganglion neurons with beta 3 tubulin antibody but I have autofluroscence problem with the background and I should do tracing for neurites of neurons and...

27 February 2022 7,894 2 View

What is the best method for purification of rat dorsal root ganglion neurons ????

Hallo everybody, Could you please, help me? I am working with dorsal root ganglion neurons and I used many methods to have pure neurons but I doesnot work with me. firstly, I used antimitotic...

15 September 2021 1,746 7 View

Could you please anybody here help me as I have a problem in attachment of dorsal root ganglion neurons on coverslips?

I usually coated the coverslips with PLL with conc 1 % and the DRGs neurons did not attach well to coverslips and I do not know why. Also , if somebody know a method for fixation of this neurons...

08 March 2021 5,824 3 View